Figure 2.

Differences in CD8⁺ T cell activation status and frequency of the T cell subpopulations in TDLNs after iPSC vaccine and control treatments

(A) t-Distributed stochastic neighbor embedding (tSNE) visualization of FlowSOM-generated clusters (live CD45⁺ cells) in merged data from cells in draining lymph nodes from mice treated with PBS, CpG alone, iPSCs alone, or the C + I vaccine. CD8⁺ T and CD4⁺ T cells were manually gated and overlaid on tSNE based on marker expression.

(B) FlowSOM results from one representative mouse treated with PBS (left panel) or the C + I vaccine (right panel) as minimum spanning trees. FlowSOM was performed using 225 clusters and 10 metaclusters. Each cluster is represented by 1 pie chart, and metaclusters are denoted by background shading.

(C) Percentage of CD8⁺ T cells among CD45⁺ cells in TDLNs from mice treated with PBS, CpG, iPSCs, or the C + I vaccine (n = 3, mean ± SEM, ^∗p < 0.05, compared with PBS, Dunnett's multiple comparison test).

(D) Percentage of activated CD69⁺CD8⁺ T cells among CD45⁺ cells in TDLNs from mice treated with PBS, CpG, iPSCs, or the C + I vaccine (left). Percentages of IFN-g⁺CD8⁺ and IL-2⁺CD8⁺ T cells among CD8⁺ T cells in TDLNs from mice treated with PBS, CpG, iPSCs, or the C + I vaccine (middle and right) (n = 3, mean ± SEM, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 compared with PBS, Dunnett's multiple comparison test).