Figure 3.

Increased activation in multiple immune cell types and decreased immune suppression after the iPSC vaccine in mice

(A and B) Spanning-tree progression analysis of density-normalized events analysis of live CD45⁺ cells in TDLNs from mice treated with PBS and the C + I vaccine with the fold-change intensity of IFN-γ⁺ and IL-2⁺ cells indicated as the color. Frequencies of IFN-γ⁺ and IL-2⁺CD45⁺ cells of total live CD45⁺ cells (n = 3, means ± SEM, ^∗p < 0.05 compared with PBS, Student's t test).

(C) Percentage of CD4⁺CD25⁺FOXP3⁺ Treg cells among CD4⁺ T cells in TDLNs and spleen from mice treated with PBS, CpG, iPSCs, or the C + I vaccine.

(D) Percentage of IL-17⁺CD4⁺ T cells among CD4⁺ T cells in TDLNs (right) from mice treated with PBS, CpG, iPSCs, or the C + I vaccine (n = 3–4, mean ± SEM, ^∗p < 0.05 compared with PBS, Tukey's multiple comparison test).