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. 2021 May 20;16(6):1598–1613. doi: 10.1016/j.stemcr.2021.04.016

Figure 2.

Figure 2

Old HSCs exhibited impaired reconstitution potential in young recipients

(A) Schematic of transplantation of HSCs from young or old mice into young recipient mice.

(B) Old HSCs contributed to donor-host-chimerism with lower efficiency compared with young HSCs. Analysis of donor-derived mature cells in PB of recipients presented as percent donor chimerism.

(C) Old HSCs produced fewer mature cells compared with young HSCs. Reconstitution data from (B) replotted as absolute numbers of donor-derived GMs, B cells, T cells, erythroid cells, and platelets per microliter of PB.

GM, granulocytes/myelomonocytes. YY, Young HSCs transplanted into young recipients; OY, Old HSCs transplanted into young recipients. Data are representative means ± SEM from nine recipient mice of young HSCs (four independent experiments) and 22 recipients of old HSCs (five independent experiments). p values were determined using unpaired two-tailed t test. #p < 0.06, ∗p < 0.05, ∗∗p < 0.005, ∗∗∗p < 0.0005.