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. 2021 May 20;16(6):1598–1613. doi: 10.1016/j.stemcr.2021.04.016

Figure 4.

Figure 4

Old HSCs exhibited impaired reconstitution potential compared to young HSCs in old recipients

(A) Schematic of transplantation of HSCs from young or old mice into separate cohorts of old mice.

(B) Old HSCs displayed lower donor-host-chimerism compared with young HSCs. Analysis of donor-derived mature cells (GMs, B cells, T cells, erythroid cells, and platelets) in PB of recipients presented as percent donor chimerism.

(C) Old HSCs produced fewer total mature cells in old recipients compared with in young HSCs. Reconstitution data from (B) replotted as absolute number of donor-derived GMs, B cells, T cells, erythroid cells, and platelets per microliter of PB.

YO, Young HSCs transplanted into old recipients; OO, Old HSCs transplanted into old recipients. Data are representative means ± SEM from 11 recipient mice of young HSCs (four independent experiments) and eight recipients of old HSCs (three independent experiments). p values were determined using unpaired two-tailed t test. ∗p < 0.05, ∗∗p < 0.005, ∗∗∗p < 0.0005.