Figure 6.

miR-92a/b and miR-541 function in mouse and human cortical cells

(A) Outline of the in vitro assay of miR-541 inhibition by locked nucleic acid (LNA)-antisense oligonucleotide lipofection in corticalized mESCs (n = 3 independent experiments) or hiPSCs (n = 3 independent experiments).

(B) Immunocytodetection shows SATB2-positive nuclei 2 days after mESC lipofection and 6 days after hiPSC lipofection, respectively.

(C) Box plots report SATB2-positive nuclei proportion. Ctr, scrambled sequence LNA lipofection. An anti-miR-92a/b LNA oligonucleotide was used to inhibit both miR-92a and miR-92b, which share the seed sequence.

(D) Mean-difference plot showing log-fold change (M) and the absolute value of the difference in mRNA expression (D) between antago-miR-541 and control antago-miR transfections (n = 3 independent experiments).

(E) Venn diagram showing the distribution of the genes differentially expressed after antago-miR transfection in the four most enriched GO terms.