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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Metab Eng. 2020 Mar 26;60:37–44. doi: 10.1016/j.ymben.2020.03.009

Fig. 3. In vitro biosynthesis of valinomycin using CFME system.

Fig. 3.

(A) SDS-PAGE (7.5% gel) analysis of Vlm1 (370 kDa) and Vlm2 (284 kDa) overexpressed individually in E. coli BAP1. M, protein molecular weight marker. (B) CFME reactions for valinomycin production by mixing two cell lysates. Error bar represents standard deviation (S.D.) of at least three independent experiments.