Fig. 3.

EC93 expresses two functional CDI systems on M9Glu+casAA solid medium. (a) Average CIs of inhibitor (EC93) and target (EC93 cdi-1 and cdi-2 mutants) cells after 16 h of co-culture on M9Glu+casAA solid medium (n=3 biological replicates). Target cells were transformed with pCDF empty vector or pCDF vector expressing either CdiI1 or CdiI2. (b) Same co-culturing experiment as in Fig. 3a but with MG1655 as target and EC93 or EC93 cdi mutants as inhibitors (n=3 biological replicates ±sem). Statistical significance was determined by two-tailed, unpaired t-test (*P<0.05).