Fig. 2.

Overview of the characterization of the validation samples. Boxes with blue headers represent different steps in the validation. The number of samples, isolates or observations is indicated at the bottom of each box. The top part of the figure represents the collection of the validation samples from the Belgian NRC and NRL for STEC. The grey boxes group the different steps of the validation: characterization with molecular methods (‘Molecular’), whole genome sequencing (‘WGS’) and in silico characterization for assays without reference information from molecular methods (‘In silico’). All detected AMR genes with WGS were confirmed to be present with PCR. National Reference Centre (NRC), National Reference Laboratory (NRL), whole genome sequencing (WGS), ampicillin (AMP), cefotaxime (CTF). *Does not include observations from 10 negative control samples from species other than E. coli .