Table 5.

P. aeruginosa proteomic expression in vivo in comparison to in vitro conditions

Square colour indicates protein expression: up-regulation (red), down-regulation (green), undetermined (light grey). (a) Protein expression in P. aeruginosa populations from CF sputa, in comparison to populations grown in vitro [101]. The 15 proteins identified with a convergent pattern within the most samples were selected. (b) Protein expression in P. aeruginosa CF isolates in comparison to PAO1 determined in vitro in minimal medium M9 [99], rich medium LB [98, 102, 103] or in sputum-like media SCFM [99] or ASMDM (artificial sputum medium with high molecular mass DNA and mucin) [103], for the 15 proteins identified as expressed in vivo. NA, Not available.

Protein name	PAO1 locus	Product	(a) In vivo vs in vitro			(b) In vitro vs PA01
			Expression in CF sputa	No. of samples with convergent pattern	No. of samples with detected protein	Expression in minimal medium	Expression in rich medium	Expression in sputum-like media
OprD	PA0958	Outer-membrane porin precursor		20	25
OprH	PA1178	PhoP/Q and low Mg2+ inducible outer-membrane protein H1 precursor		27	33
PA1288	PA1288	Probable outer-membrane protein precursor		26	33
OprI	PA2853	Outer-membrane lipoprotein OprI precursor		26	35
AlgE	PA3544	Alginate production outer-membrane protein AlgE precursor		20	21
FumC1	PA4470	Fumarate hydratase		24	30
PhuR	PA4710	Haem/haemoglobin uptake outer-membrane receptor precursor		22	32
PA4793	PA4793	Hypothetical protein		23	31
PA4837	PA4837	Probable outer-membrane protein precursor		28	31
Hfq	PA4944	Hfq		19	29
PstS	PA5369	Phosphate ABC transporter, periplasmic phosphate-binding protein		25	26
NA	NA	TonB-dependent receptor		24	25
Icd	PA2623	Isocitrate dehydrogenase		21	30
RpsB	PA3656	30S ribosomal protein S2		20	28
RplS	PA3742	50S ribosomal protein L19		23	26