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. Author manuscript; available in PMC: 2021 Jun 10.
Published in final edited form as: J Leukoc Biol. 2020 May 9;108(3):983–998. doi: 10.1002/JLB.1A0420-012RR

Figure 1. Phenotype of peripheral blood monocytes before and after differentiation stimulated by Trophoblast CM.

Figure 1.

A. Gating strategy was set up for characterization of circulating monocytes. Isolated peripheral blood CD14+ monocytes express CD14 and CD86 but not CD206 (CD14+/CD206−/CD86+)

B. Freshly isolated CD14+ monocytes were exposed to first trimester trophoblast CM for 6 days and then analyzed by flow cytometry for CD14, CD86, CD206 and CD16. Following treatment with trophoblast CM, monocytes/macrophages maintained CD14 expression, and gained CD16 and CD206, but lost CD86 (CD14+/CD16+/CD206+ /CD86−). Representative figure of 6 independent experiments with each done in triplicate.