Figure 1.
PBMT induces hair regeneration
(A) Postnatal mouse hair cycle and PBMT. PBMT on the back skin every day for 14 consecutive days starting on the 50th day after birth (635 nm, 6.67 mW/cm2, 20 min).
(B) PBMT induced telogen-anagen transition, as shown by the dorsal skin turning from pink to black, n = 12 mice.
(C) H&E images of the dorsal skin of mice at the indicated time point. n = 8 mice per group and >20 sections. ∗p < 0.05; ∗∗∗p < 0.001.
(D) The expression of LEF1 in HFs was detected by confocal microscopy on the seventh day of PBMT, n = 8 mice per group and >60 HFs per mouse.
(E and F) The BrdU incubation experiment detected the proliferation of HFSCs by confocal (E) and flow cytometry (F), n = 8 mice.
(G and H) PBMT on the old mouse back skin every day for 1 month starting on the 16 months after birth (635 nm, 6.67 mW/cm2, 20 min), n = 8 mice.
(I) Hair density of old mice after treatment with PBMT for 1 month, n = 8 mice.
(J) H&E images of the dorsal skin of old mice after PBMT on 1 month, n = 8 mice per group and >20 sections.
(K) Old mice after treatment with PBMT for 1 month. Flow cytometry was used to detect the proliferation of HFSCs, n = 6 mice.
(L) Tape analysis. The surgical tape was attached to the back hair of the mouse, and then it was peeled off to observe the number of hairs that fell through the tape, n = 6 mice. ANOVA was used for significance test. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.