Figure 3.

Activation of PI3K/AKT/GSK-3β signaling pathway by PBMT stabilizes β-CATENIN

(A) Representative western blot was performed to detect phosphorylation levels of AKT and GSK3 and β-CATENIN expression in HFSCs with or without PBMT.

(B) In the presence of API-2 and wortmannin, HFSC was stimulated with PBMT, and representative AKT and GSK3 phosphorylation levels and β-CATENIN protein levels were detected by western blot.

(C) Representative western blot assay for detecting the effect of PBMT on p-β-CATENIN (Ser552) and β-CATENIN (Ser45) proteins expression in HFSCs.

(D) Representative immunofluorescent images of β-CATENIN in HFSCs, n = 10 mice. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). All the data represent mean ± SD, n = 3 independent replicates. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗ p < 0.001.