Figure 4.

PBMT activates PI3K/AKT signaling in HFSCs by inducing ROS production

(A and B) HFSCs labeled with 10 μM DCF-diacetate were treated with PBMT, and the fluorescence changes were detected by flow cytometry and confocal analysis after 30 min. The data represent mean ± SD, n = 3 independent replicates. ^∗∗ p < 0.01; ^## p < 0.01.

(C) Western blot analysis of β-CATENIN protein levels under different concentrations of H₂O₂. The data represent mean ± SD, n = 3 independent replicates. ^∗ p < 0.05.

(D) Western blot analysis of oxidized and reduced PTEN levels. The data represent mean ± SD, n = 3 independent replicates. ^∗∗∗ p < 0.001.

(E) Representative western blot was performed to detect p-AKT, P-GKS-3β, and β-CATENIN levels under H₂O₂ and PBMT. The data represent mean ± SD, n = 3 independent replicates. ^∗∗ p < 0.01; ^∗∗∗ p < 0.001.

(F) Representative western blot analysis p-AKT, P-GKS-3β, and β-CATENIN levels of HFSCs after PBMT in the presence of NAC; the addition of exogenous H₂O₂ was used as a positive control. The data represent mean ± SD, n = 3 independent replicates. ^∗∗p < 0.01; ^∗∗∗p < 0.001.

(G) Representative immunofluorescent images of β-CATENIN in HFSCs. Nuclei were stained with DAPI. The data represent mean ± SD, n = 3 independent replicates. ^∗∗∗p < 0.001.

(H and I) Representative immunofluorescent images of β-CATENIN (H), P-AKT, and p-GSK3β (I) in HFs, n = 8 mice per group and >60 HFs per mouse. ANOVA was used for significance test. ^∗p < 0.05.