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. 2021 Jun 10;23:166. doi: 10.1186/s13075-021-02464-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — MTX down-regulates ACE2 expression. a Real-time PCR showing quantification of mRNA for ACE2 in the various segments off the intestine and the lung in mice treated with methotrexate (MTX; N = 3 per group per experiment; data summarized from two independent experiments). b Representative immune histochemistry staining for ACE2 (brown color, arrowheads) in the intestine and the lung of mice treated with methotrexate or vehicle for 3 days. Isotype control antibody staining is also shown. c Quantification of ACE2 expression in immune histochemistry (N = 3 per group per experiment; data summarized from two independent experiments). d Representative immunoblot for ACE2 and actin (control) in intestinal organoids treated with two doses of methotrexate including densitometric quantification