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. 2021 Jun 10;31(8):847–860. doi: 10.1038/s41422-021-00519-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Single-channel current recording of 2-E after reconstitution in lipid bilayers with PC/PS = 3:2 lipids at the indicated potentials and solutions. Protein (5–50 ng/mL) was added to the cis side. All-point current histograms for the left trace (0, 0, –50, 0, 0, 0, 0 mV) (“C” means Closed; “O” means Open). b I-V curves of 2-E proteins in different solutions as the left panels (n ≥ 3). c Representative single-channel current recordings of 2-E in the presence of various pH in 0 mV. Right showed the amplitude histograms by Gaussian fit. The currents were recorded in asymmetric 50:500 mM KCl solutions (trans:cis) and the pH of both cis and trans sides were changed at the same time. d I–V plot (Left) and open probability (Right) were shown in different pH conditions (n ≥ 3). e Schematic diagram of 2-E channel open and closed states. The reverse potential and permeability of each ion was shown. All error bars are SEM.