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. 2021 Jun 10;31(8):847–860. doi: 10.1038/s41422-021-00519-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Cell viability of 14 cell lines at the indicated time after transfection with 2-E plasmids. b Microscopy images of 2-E expression in Vero E6 cells during cell death (bar, 10 μm). c Images of Vero E6 cells infected with SARS-CoV-2 virus (bar, 25 μm). Vero E6 and Calu-3 cells were transfected with 2-E-mCherry plasmid at indicated concentration. d Survival viability of Vero E6 cells under different transfection levels. e Protein expression levels of 2-E in Vero E6 cells after transfection. f Expression of 2-E present in 2-E-transfected and SARS-CoV-2-infected Vero E6 (left) and Calu-3 (right) cells (MOI = 0.1). All data are representative of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; unpaired Student’s t-test. All error bars are SEM.