Figure 4.

eIF4E was the target of miR-768-3p in breast cancer. (A) Schematic diagram of binding sites between eIF4E mRNA 3′UTR sequence and miR-768-3p was performed using TargetScan v.7.2. The binding sites were linked by short vertical lines. (B) Luciferase reporter assay was used to determine the targeted binding of miR-768-3p and eIF4E. (^###P<0.001, compared with mimic NC; ^&&&P<0.001, compared with inhibitor NC). miR, microRNA; RT-q, reverse transcription-quantitative; NC, negative control; WT, wild-type; Mut, mutant; EIF4E, eukaryotic translation initiation factor 4E; control, untransfected cells.