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. 2021 Jun 9;20:115. doi: 10.1186/s12934-021-01605-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Pilot-scale fermentation, purification and characterization of IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. a Representative three-step fermentation process, including batch phase, glycerol fed-batch phase and methanol induction phase. Parameters, such as dissolved oxygen (DO), feeding speed, temperature and pH, were monitored during fermentation. b Cell growth of the two IL-15/SuIL-15Rα-IgG4 Fc complex-expressing strains during fermentation was monitored and represented as wet cell weight. c The expression of the two IL-15/SuIL-15Rα-IgG4 Fc complexes during fermentation was analyzed by Western blotting using an anti-human IgG4-HRP conjugate under nonreducing conditions. d The downstream processing workflow of fermentation broth. e The two purified IL-15/SuIL-15Rα-IgG4 Fc complexes were separated by SDS-PAGE under reducing conditions and identified by Western blotting or Coomassie blue staining. The contents within the dashed boxes are the presumed protein structure of the corresponding bands. M: prestained protein marker; dFc: IL-15/SuIL-15Rα-dFc; mFc: IL-15/SuIL-15Rα-mFc