Table 2.
Effects of rhIL-2, rhIL-15 and IL-15/SuIL-15Rα-IgG4 Fc complexes on NK cell phenotypic markers
| Marker | Ctrl | rhIL-2 | rhIL-15 | dFc | mFc |
|---|---|---|---|---|---|
| CD69 | 5.8 ± 3.0 | 28.3 ± 13.9 | 81.4 ± 18.6 | 85.4 ± 11.8 | 78.6 ± 22.0 |
| CD25 | 2.9 ± 0.6 | 3.5 ± 0.5 | 24.0 ± 8.6 | 24.6 ± 7.8 | 22.7 ± 8.2 |
| CD94 | 65.6 ± 29.0 | 73.4 ± 22.2 | 86.2 ± 10.6 | 84.8 ± 12.1 | 85.2 ± 11.6 |
| NKG2D | 75.6 ± 12.8 | 91.6 ± 5.4 | 96.1 ± 2.0 | 95.6 ± 2.7 | 95.7 ± 2.5 |
| NKp44 | 2.3 ± 0.6 | 5.0 ± 1.5 | 9.2 ± 3.8 | 12.0 ± 3.6 | 8.9 ± 3.4 |
| CD107a | 6.4 ± 2.1 | 11.2 ± 6.4 | 20.3 ± 9.7 | 21.7 ± 12.0 | 21.2 ± 7.9 |
| IFN-γ | 1.4 ± 0.5 | 2.1 ± 0.7 | 10.0 ± 2.7 | 10.8 ± 2.1 | 11.7 ± 3.8 |
| TRAIL | 20.0 ± 14.8 | 34.0 ± 13.4 | 60.1 ± 17.1 | 62.7 ± 15.4 | 60.8 ± 18.1 |
| 4-1BB | 2.3 ± 0.9 | 6.2 ± 3.6 | 15.3 ± 6.9 | 16.9 ± 7.8 | 15.0 ± 7.6 |
| Granzyme B | 89.7 ± 4.4 | 96.3 ± 1.6 | 98.8 ± 0.8 | 98.9 ± 0.8 | 98.8 ± 0.9 |
| Ki67 | 4.9 ± 1.7 | 7.3 ± 2.5 | 16.2 ± 2.6 | 16.6 ± 2.8 | 16.0 ± 2.2 |
| Tim-3 | 9.0 ± 4.8 | 33.5 ± 11.7 | 57.1 ± 13.7 | 58.4 ± 15.4 | 55.4 ± 15.7 |
| TIGIT | 29.3 ± 2.8 | 40.0 ± 5.9 | 55.4 ± 11.0 | 56.0 ± 7.9 | 55.7 ± 10.5 |
Human PBMCs from healthy donors (n = 5) were cultured for 24 h in the absence or presence of 50 IU/mL rhIL-2, 10 ng/mL rhIL-15 or 35.7 ng/mL IL-15/SuIL-15Rα-IgG4 Fc complexes, and the expression of selected markers on NK cells was analyzed by flow cytometry. The percentages of NK cells that expressed the corresponding markers are listed in the table. dFc: IL-15/SuIL-15Rα-dFc; mFc: IL-15/SuIL-15Rα-mFc