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. Author manuscript; available in PMC: 2021 Jun 10.
Published in final edited form as: Cell Rep. 2021 Jun 1;35(9):109190. doi: 10.1016/j.celrep.2021.109190

Figure 2. SIRT6 deficiency upregulates fatty acid transporters.

Figure 2.

(A) The levels of fatty acid transporters in the hearts of SIRT6 knockout (SIRT6KO) mice measured by western blotting, n = 4 mice per group.

(B and C) Fatty acid transporters expression in the kidney (B) and muscle (C) of SIRT6KO mice, n = 3 mice per group.

(D) The levels of fatty acid transporters in the hearts of SIRT6 heterozygous mice, n = 3 mice per group.

(E) Representative confocal images showing the level of fatty acid transporters (stained green) in SIRT6-depleted and SIRT6-overexpressing primary cardiomyocytes. n = 3 independent experiments, scale bar, 20 μm. SIRT6 depletion and overexpression were confirmed by immunoblotting, as shown in Figures S2G and S2H.

(F) The levels of fatty acid transporters in the primary cardiomyocytes transfected with either vector, SIRT6-WT, SIRT6-H133Y, or SIRT6-S56Y plasmids. n = 6.

(G) Quantification of fatty acid transporter protein levels in the primary cardiomyocytes transfected with either vector, SIRT6-WT, SIRT6-H133Y, or SIRT6-S56Y plasmids. Data presented as means ± SDs, n = 6, 1-way ANOVA, *p < 0.05.