Fig. 2. Imaging processing of bioorthogonal and fluorophore-modified Ova in DC 2.4-APCs via confocal microscopy. DCs were incubated for 2 h (pulse) with Aha or A647-Ova. Cells were fixed with 0.5% PFA and processed for immunofluorescence with LAMP-1 as a lysosomal marker (green in merged images). The nucleus was stained with DAPI (blue in merged images). Aha-Ova was stained using ccHc with A647-alkyne fluorophore (grey in single channel, red in merged images). (A) Upper panel: imaging of Aha-Ova, lower panel: imaging of A647-Ova (B) screenshot from Movie S1 (ESI†). Scale bar is 10 μm (white bar, left corner).