Fig. 4. Effect of post-translational modifications on vinculin degradation. Incubation of vinculin variants with isolated lysosomes (A) or CatS (B) show decreased proteolysis of wt and Aha-Vin upon carbamylation. Vin peptides recovered after CatS digestion (n = 3 per variant) were mapped against the protein sequence. Peptide sequences were marked green, when found in at least 2/3 replicates.
