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. 2020 Jul 10;26(10):5766–5788. doi: 10.1038/s41380-020-0806-5

Fig. 4. Localisation of AβDP degradation products and Aβ peptides with BACE2 in hippocampal sections of the human post-mortem brain.

Fig. 4

a Immunofluorescence analysis of the brain of DS-AD-1 co-stained for Aβx-34, BACE2, and GFAP. A typical near-circular neuritic plaque is shown (in which DAPI faintly stains the fibrillar amyloid deposits). Arrows indicate three categories of objects in which the colocalisation of BACE2 and the AβDP product Aβx-34 is observed. White arrows: intraneuronal fine-vesicular pattern; white arrowheads: large intraneuronal spherical granules (lipofuscin); black arrows with white arrowheads: amorphous extracellular aggregates. See Methods and Supplementary Fig. 8 for experiments controlling the extent of lipofuscin autofluorescence effects. b DS-AD1 brain co-stained for Aβx-34 and BACE1, or Aβx-40, BACE2 and GFAP, and Pearson’s coefficient of colocalisation for proteins stained in parts ‘a’ and ‘b’, with the addition of the staining for Aβx-42 neo-epitope (not shown). Error bars: SEM. c Same IF staining combinations as in part ‘a’ (except for GFAP) were used in three additional brain samples: DS-AD-2, 3, and 4. d Brain sample co-stained for BACE2 and Aβx-34 of a 28 yrs old person with Down syndrome without dementia, and euploid non-demented (ND) controls aged 42 and 84. Scale bar: 20 μm.