Fig. 3.

Overexpression of CD151 promotes NSCLC cell proliferation, migration and invasion. a CD151 mRNA and protein levels in CD151-overexpressed NSCLC cell lines. b CCK-8 assay of cell viability in NSCLC cell lines. c Representative images of clonogenic analysis of cell proliferation in NSCLC cells. Bar charts showed clonogenic growth of NSCLC cells. d Flow cytometry analysis of cell cycle of NSCLC cell lines (CD151-overexpressed cells vs. Vector cells). Cells were harvested at 72 h after stained with propidium iodide. e EdU incorporation assay for cell proliferation(n = 3). EdU (red), DAPI (blue). Scale bar = 200 μm. f Wound healing assay was performed to observe the role of CD151 in A549 and H1299 cells. g Representative images of the transwell assay results for cell migration and invasion in A549 and H1299 cells (CD151-overexpressed compared with vector). β-actin was used as the internal control. Bars represent mean ± SD from three independent experiments. Significant differences compared with the control: * P < 0.05; **P < 0.01; ***P < 0.001