Fig. 3.

Percent viability of M. chelonae carrying no prophage ΔMcProf (McΔMP), BPs prophage (Mc + BPsΔMP), prophage McProf (Mc + MP) or both prophages BPs and McProf (Mc + BPs + MP) in the presence of varying concentrations of amikacin. To determine if the presence of each prophage interacts with sub-inhibitory concentrations of antibiotics, each strain was treated or not treated with 75 μM acivicin (ACI). Graphs represent average values ± SE of the mean with n = 3. The optical density of was measured at 570- and 600 nm after the addition of 2 μl of AlamarBlue and the percent difference in reduction between antibiotic-treated cells and untreated cells was calculated. Data is representative of two independent experiments