Table 1.
Primer sequence of genes for RT-qPCR
| Gene groups | Gene names | Primer Sequence (5´ → 3´) |
|---|---|---|
| Selected genes from bioinformatics analysis | DDIT4 |
F: CTTTGGGACCGCTTCTCGTC R: GGTAAGCCGTGTCTTCCTCCG |
| SULF1 |
F: GGACGGATACAGCAGGAACG R: CAGCACATGGGTGTAGTCACA |
|
| TPTEP1 |
F: AGCCGCAGACAAAAGACCTCGG R: CCACCAAACAGGCTTCGTGTGA |
|
| miRNA-181d-5p | AACATTCATTGTTGTCGGTGGGT | |
| miRNA-148b-3p | TCAGTGCATCACAGAACTTTGT | |
| Stemness genes | OCT4 |
F: GTGGAGAGCAACTCCGATG R: TGCAGAGCTTTGATGTCCTG |
| SOX2 |
F: AATGGGAGGGGTGCAAAAGAGG R: GTGAGTGTGGATGGGATTGGTG |
|
| C-MYC |
F: ACACATCAGCACAACTACG R: CGCCTCTTGACATTCTCC |
|
| KLF4 |
F: CCTCGCCTTACACATGAAGAG R: CATCGGGAAGACAGTGTGAAA |
|
| NANOG |
F: AGCTACAAACAGGTGAAGAC R: GGTGGTAGGAAGAGTAAAGG |
|
| EMT genes | TWIST1 |
F: TTCTCGGTCTGGAGGATGGAG R: ACGCCCTGTTTCTTTGAATTTGG |
| SNAIL1 |
F: CCAGAGTTTACCTTCCAGCA R: GATGAGCATTGGCAGCGA |
|
| VIM |
F: TCTACGAGGAGGAGATGCGG R: GGTCAAGACGTGCCAGAGAC |
|
| ZEB1 |
F: CTTCTCACACTCTGGGTCTTATTC R: CGTTCTTCCGCTTCTCTCTTAC |
|
| ABC transporter genes | ABCB1 |
F: GTTCAGGTGGCTCTGGATAAG R: AGCGATGACGTCAGCATTAC |
| ABCG2 |
F: TTCCACGATATGGATTTACGG R: GTTTCCTGTTGCATTGAGTCC |
|
| ABCC1 |
F: CGCCTTCGCTGAGTTCCT R: TGCGGTGCTGTTGTGGTG |
|
| Housekeeping genes | GAPDH |
F: CATGAGAAGTATGACAACAGCCT R: AGTCCTTCCACGATACCAAAGT |
| RNU6 (U6) |
F: TCGCTTCGGCAGCACATATAC R: GCGTGTCATCCTTGAGCAG |