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. 2021 May 13;296:100739. doi: 10.1016/j.jbc.2021.100739

Figure 2.

Figure 2

TRAF4 regulates IRS-1-mediated IGF-1 signaling.A, depletion of TRAF4 or IRS-1 inhibits IGF-1-induced downstream ERK and AKT phosphorylation. IRS-1 or TRAF4 was depleted in MCF-7 cells using a pool of two different siRNAs against IRS-1 or TRAF4, respectively. MCF-7 cells were then serum starved for 24 h and treated with or without IGF-1 (100 ng/ml) for 15 min. Gene knockdown efficiency and ERK and AKT phosphorylation were determined by Western blot. B, depletion of TRAF4 or IRS-1 inhibits MCF-7 cell proliferation. TRAF4 or IRS-1 was knocked down using two different TRAF4 or IRS-1 siRNAs, respectively, or transfected with control siRNA in MCF-7 cells. Cell proliferation was determined for indicated period of time using MTS assay. ∗p < 0.05 by two-way ANOVA with Tukey’s multiple comparisons test. Data are presented as mean ± SD. C, IRS-1 or TRAF4 depletion reduced IGF-1-induced cell proliferation. These MCF-7 cells transfected with control, IRS-1 or TRAF4 siRNA were serum starved and treated with or without IGF-1 followed by cell proliferation assay. Cell proliferation was determined for indicated periods using an MTS assay. ∗p < 0.05 by two-way ANOVA with Tukey’s multiple comparisons test. Data are presented as mean ± SD.