Figure 7.

WNL-mVP40 mutant accumulates in intracellular structures. TEM micrographs of zoomed intracellular structures EGFP-mVP40 (WT or WNL) coexpressed with GBP-APEX2 in (A), while (B) and insets (C and D) show intracellular accumulations of WNL protein in cells. Quantification of the average number of virus-like particles (VLP) per μm² of cell surface and VLP length and diameter measurements performed on TEM micrographs are shown in (E–G), respectively. These analyses were performed as described in Figure 3. Values are reported as mean ± SD of three independent means. One-way ANOVA with multiple comparisons were performed (∗∗p < 0.005, ∗∗∗∗p < 0.0001). H, the chromatogram of gel filtration analysis of protein extract from HEK293 cells transfected with EGFP-WT-mVP40 shown as absorbance (280 nm) versus elution volume. Molecular mass standard curve is plotted in (I) as log values of molecular weights versus elution volume. J, Western blot analyses of each protein are indicated. EGFP empty vector served as a negative control. CL, cell lysate.