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Kinetics of 4MU-Glc hydrolysis
| rhGAA form | Vmax ± SD (nmol/nM GAA/h) | Km ± SD (μM) | Vmax/Km |
|---|---|---|---|
| rhGAA precursor | 1.2 ± 0.15 | 1709 ± 350 | 0.000684 |
| rhGAA–AOAA precursor | 1.1 ± 0.29 | 1516 ± 520 | 0.000729 |
| rhGAA–kif precursor | 1.2 ± 0.38 | 1741 ± 722 | 0.000685 |
| rhGAA processed | 1.2 ± 0.02 | 1903 ± 31 | 0.000643 |
| rhGAA–AOAA processed | 1.3 ± 0.58 | 2036 ± 1130 | 0.000636 |
| rhGAA–kif + Endo H | 1.1 ± 0.01 | 1715 ± 542 | 0.000669 |
