Figure 2.
Transcriptome zonation defines distinct L-EC signatures
(A) Heatmap representation of the expression profiles of 4,943 genes with significantly zonated expression. Genes are normalized to their maximum expression and sorted by their center-of-mass. Representative central and portal zonated genes are indicated in blue and red, respectively (n = 4).
(B) Expression profiles of 890 genes significantly zonated on large vessels or sinusoids. Genes are normalized to their maximum expression and sorted by their log2 fold change of vessel to sinusoid. Representative vessel and sinusoidal zonated genes are indicated in orange and green, respectively (n = 4).
(C) Representative gene expression profiles for each zonation pattern (as indicated by color) with the corresponding qRT-PCR validation (black). Gene expression is represented by percentage of maximum; patches represent SD (n = 4).
(D) Representative liver immunofluorescence staining for large vessel and sinusoidal zonation patterns. CD31 in gray represents large vessel zonation and Lyve-1 in red represents sinusoidal zonation. Scale bars, 100 μm (left), 50 μm (right).
(E) Expression profiles of representative transcription factors zonated in the L-ECs. Gene expression is represented by percentage of maximum; patches represent SD (n = 4); color scale indicates relative expression of individual samples as in (A).
See also Figures S2 and S3.