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. 2021 May 17;49(10):5956–5966. doi: 10.1093/nar/gkab370

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — RdRP assay using a hairpin RNA substrate. (A) Schematic structure of the hairpin RNA. (B) Time-dependent incubation of NSP7, NSP8 and/or NSP12 with RNA template. (C) Time-dependent incubation of NSP7, NSP8 and NSP12 with RNA template, with NSP7 or NSP8 mutated at interface I. TM refers to NSP7 F49A/M52A/L56A triple mutation. (D) Time-dependent incubation of NSP7, NSP8 and NSP12 with RNA template, with NSP7 or NSP8 mutated at interface II. (E) Time-dependent incubation of NSP7, NSP8 and NSP12 with RNA template, with NSP8 mutated at the N-terminal domain.