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. 2021 May 12;49(10):5520–5536. doi: 10.1093/nar/gkab339

Figure 2.

Figure 2.

Accumulation of unspliced transcripts upon nuclear depletion of FRB-tagged Rat1 in the presence of rapamycin. (A) Schematic depiction of a gene showing the position of primers F and R used in RT-PCR analysis. (B) Gel pictures showing RT-PCR products for the indicated genes in FRB-tagged Rat1 strain and untagged strain (control) in the presence and absence of rapamycin. (C) Quantification of data in lanes 3 and 4 of (B). 5S rRNA was used as normalization control. The results shown are an average of six independent PCRs from three separate RNA preparations from three independently grown cultures. The values and error bars for each condition indicate the mean ± standard deviation. Statistical significance (P-values) was determined using the two-tailed paired Student's t-test as described in (58). (D) Splicing efficiency representing the percentage of total transcripts undergoing splicing in FRB-tagged Rat1 strain and untagged (control) strain in the presence and absence of rapamycin.