Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 28;49(10):5637–5653. doi: 10.1093/nar/gkab401

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 6. — (A) TbTRF binds to single stranded UUAGGG repeats directly. EMSA was performed using the recombinant GST-tagged TbTRF_2-382 (numbers indicate aa positions) expressed from E.coli (41) and ss(UUAGGG)₁₂ or ss(CCCUAA)₁₂ as the probe. Non-radiolabeled (UUAGGG)₁₂ was added as a competitor. The amount of TbTRF (ng) used is indicated on top of each lane. (B, C) TbTRF associates with TERRA in vivo. Cell lysates were incubated with TbTRF antibody (41) (B) or a rabbit anti-GFP antibody (ThermoFisher) (C). RNA was isolated from the IP products and analyzed by slot blot hybridization with a (CCCTAA)_n-containing probe. A representative slot blot is shown at the top. Hybridization with an rRNA probe is used as a negative control in (B). The average enrichment of the RNA-IP product was calculated from three slot blot hybridizations and is shown at the bottom. P values of unpaired t-tests are shown in (B) and (C).