Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 21;49(10):5743–5759. doi: 10.1093/nar/gkab381

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 6. — Depletion of Rcl1 causes down-regulation of 1344 genes and up-regulation of 1199 gene. (A) Sequencing analysis of WT rcl1 and mutant rcl1^zju1 transcripts in the RT-PCR products obtained from WT, rcl1^zju1/+ heterozygous and rcl1^zju1 homozygous progenies of the rcl1^zju1/+ intercross at 3.5dpf, 4.5dpf and 5.5dpf. (B) Hierarchical Clustering analysis of the RNA-seq data between WT and rcl1^zju1 mutant with three biological repeats. (C) The log₂ value distributions of the 1,199 significant up-regulated and 1,344 down-regulated genes (cut-off value: log₂≧|, P <0.05) in rcl1^zju1 mutant at 4.5dpf. (D) Confirmation of the RNA-seq data by the qPCR analysis of 20 randomly selected genes in WT and rcl1^zju1 mutant. Expression data of genes detected by RNA-seq was plotted against those by qPCR. The reference line indicates the linear correlation between the RNA-seq and qPCR. Blue dot: gene with significant change in RNA-seq; red dot: gene without significant change.

Inline graphic — Depletion of Rcl1 causes down-regulation of 1344 genes and up-regulation of 1199 gene. (A) Sequencing analysis of WT rcl1 and mutant rcl1^zju1 transcripts in the RT-PCR products obtained from WT, rcl1^zju1/+ heterozygous and rcl1^zju1 homozygous progenies of the rcl1^zju1/+ intercross at 3.5dpf, 4.5dpf and 5.5dpf. (B) Hierarchical Clustering analysis of the RNA-seq data between WT and rcl1^zju1 mutant with three biological repeats. (C) The log₂ value distributions of the 1,199 significant up-regulated and 1,344 down-regulated genes (cut-off value: log₂≧|, P <0.05) in rcl1^zju1 mutant at 4.5dpf. (D) Confirmation of the RNA-seq data by the qPCR analysis of 20 randomly selected genes in WT and rcl1^zju1 mutant. Expression data of genes detected by RNA-seq was plotted against those by qPCR. The reference line indicates the linear correlation between the RNA-seq and qPCR. Blue dot: gene with significant change in RNA-seq; red dot: gene without significant change.