Fig 6. Entrainment of OFA with restricted food availability during the subjective day (RF).

A. Activity profiles of mice entrained to LD12:12 cycles. Left panels: group-accumulated actograms of WT (n = 10) and mPer2^Luc/Luc (n = 25) mice calculated as activity average (upper pair) or median (lower pair). The short activity bout (red arrow) during the last inactive period (at ZT9) is the reaction to the first food removal at the beginning of the RF schedule. Right panels: group-composite daily activity profiles for 10 animals/genotype over the interval shown to the left. B. Representative double-plotted activity records from WT and mPer2^Luc/Luc animals in LD and exposed to RF for 10 days (green area). For each animal the raw activity recordings are shown in the actogram on the left, and the average activity profiles for the 1^st three days and last three days of RF are shown on the right. C. Representative single plotted actograms (from records in B, No.1 WT and No. 1 mPer2^Luc/Luc) demonstrate genotype specific differences in the mode of behavioral adjustment to RF. The end of activity (WT) or beginning of the late-night activity bout (mPer2^Luc/Luc) are depicted day-by-day as the red stars. In mPer2^Luc/Luc animals the late-night activity bout, but not the early activity bout, gradually entrains with the food timing. In WT mice the activity adjusts as published elsewhere.⁶⁷ D. Total activity during ad libitum feeding did not differ between genotypes but was significantly higher in WT during RF. **** = p < .0001.