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. 2021 Jun 10;17(6):e1009616. doi: 10.1371/journal.ppat.1009616

Fig 3. P300 regulates the release of VP40 VLPs.

Fig 3

(A) HEK293T cells were transfected with increasing doses of P300-HA (0 μg, 1 μg and 3 μg) to detect its influence on VP40 egress. (B) The release of VP40 VLPs was measured using electron microscopy in HEK293T WT cells, P300 KO cells and NEDD4 KO cells. Scale bar, 500nm. (C)–(F) The release of VP40 VLPs was measured by overexpressing P300 acetyltransferase-dead mutant P300 WY-HA in P300 KO cell lines (C); by refreshing P300 and P300-WY levels in P300 KO cells (D); by overexpressing P300-HA in HEK293T NEDD4 KO cells (E); by overexpressing P300-HA, the NEDD4 WT and the NEDD4K667R mutant in HEK293T NEDD4 KO cells (F). Error bars, mean ± SD of three experiments. Student’s t test; *p < 0.05; **p < 0.01; ***p < 0.001.