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(A, B) Proteins in the lysate of R. typhi were digested with Proteinase K for indicated periods. Control bacterial lysate was left undigested. (A) A Western Blot and (B) a Dot blot was performed. The membranes were incubated with the BNI52 antibody followed by the incubation with a HRP-labeled secondary antibody. The antigen was no longer detectable after 24h proteinase K digestion.
