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. Author manuscript; available in PMC: 2021 Jun 10.
Published in final edited form as: Hum Pathol. 2018 Dec 8;86:66–75. doi: 10.1016/j.humpath.2018.11.020

Table 2.

Used antibody, clone, company, dilution, and scoring

Antibody Clone Company Dilution (incubation time) Scoring
SSTR1 sstr1 (monoclonal) AbD Serotec, Oxford, United Kingdom 1:500 (30 min) 0, 0; 1, <10%, weak cytoplasmic positivity; 2, ≥10% weak cytoplasmic positivity; 3, moderate cytoplasmic positivity; 4, strong cytoplasmic positivity a
SSTR2 UMB-1 (monoclonal) Abcam 1:300 (30 min) 0, 0; 1, <10%, not totally circumferential membrane positivity; 2, ≥10% not totally circumferential membrane positivity; 3, strong circumferential membrane positivity in 10%-94% of tumor cells; 4, strong circumferential positivity in ≥95% of tumor cells b
SSTR3 UMB-5 (monoclonal) Abcam 1:7000 (60 min) Intensity of cytoplasmic staining
0, 0; 1, weak; 2, moderate; 3, strong
If <10% of tumor cells positive, intensity downgraded by 1 grade c
SSTR4 sstr4 (monoclonal) AbD Serotec, Oxford, United Kingdom 1:500 (30 min) Same as SSTR1
SSTR5 sstr5 (monoclonal) Bio-Rad, Oxford, United Kingdom 1:1000 (30 min) 0, 0; 1, <10%,weak cytoplasmic positivity; 2, ≥10% weak cytoplasmic positivity; 3, moderate cytoplasmic or some membrane positivity; 4, strong cytoplasmic positivity or membrane positivity a
Ki-67 MIB1/M7240 (monoclonal) Agilent 1:100 (60 min) Percentage of positive cells
Chromogranin A Rabbit polyclonal Agilent 1:2000 (30 min) Positive/negative
SDHB 21A11 (mouse monoclonal) Abcam 1:1000 Positive/negative
SDHA 5A11 (mouse monoclonal) Abcam 1:1000 Positive/negative
a

For statistics, the scores for SSTR1, SSTR4, andSSTR5 were grouped into 3: negatives (score 0 and 1), intermediate positive (scores 2 and 3), and strongly positive (score 4).

b

For statistics, the SSTR2 scores were grouped into 3: negative (scores 0 and 1), intermediate positive (score 2), and strongly positive (scores 3 and 4).

c

For statistics, the SSTR3 scores were grouped into 3: negative (score 0), intermediate positive (score 1), and strongly positive (scores 2 and 3).