Table 2.
Antibody | Clone | Company | Dilution (incubation time) | Scoring |
---|---|---|---|---|
SSTR1 | sstr1 (monoclonal) | AbD Serotec, Oxford, United Kingdom | 1:500 (30 min) | 0, 0; 1, <10%, weak cytoplasmic positivity; 2, ≥10% weak cytoplasmic positivity; 3, moderate cytoplasmic positivity; 4, strong cytoplasmic positivity a |
SSTR2 | UMB-1 (monoclonal) | Abcam | 1:300 (30 min) | 0, 0; 1, <10%, not totally circumferential membrane positivity; 2, ≥10% not totally circumferential membrane positivity; 3, strong circumferential membrane positivity in 10%-94% of tumor cells; 4, strong circumferential positivity in ≥95% of tumor cells b |
SSTR3 | UMB-5 (monoclonal) | Abcam | 1:7000 (60 min) | Intensity of cytoplasmic staining 0, 0; 1, weak; 2, moderate; 3, strong If <10% of tumor cells positive, intensity downgraded by 1 grade c |
SSTR4 | sstr4 (monoclonal) | AbD Serotec, Oxford, United Kingdom | 1:500 (30 min) | Same as SSTR1 |
SSTR5 | sstr5 (monoclonal) | Bio-Rad, Oxford, United Kingdom | 1:1000 (30 min) | 0, 0; 1, <10%,weak cytoplasmic positivity; 2, ≥10% weak cytoplasmic positivity; 3, moderate cytoplasmic or some membrane positivity; 4, strong cytoplasmic positivity or membrane positivity a |
Ki-67 | MIB1/M7240 (monoclonal) | Agilent | 1:100 (60 min) | Percentage of positive cells |
Chromogranin A | Rabbit polyclonal | Agilent | 1:2000 (30 min) | Positive/negative |
SDHB | 21A11 (mouse monoclonal) | Abcam | 1:1000 | Positive/negative |
SDHA | 5A11 (mouse monoclonal) | Abcam | 1:1000 | Positive/negative |
For statistics, the scores for SSTR1, SSTR4, andSSTR5 were grouped into 3: negatives (score 0 and 1), intermediate positive (scores 2 and 3), and strongly positive (score 4).
For statistics, the SSTR2 scores were grouped into 3: negative (scores 0 and 1), intermediate positive (score 2), and strongly positive (scores 3 and 4).
For statistics, the SSTR3 scores were grouped into 3: negative (score 0), intermediate positive (score 1), and strongly positive (scores 2 and 3).