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. Author manuscript; available in PMC: 2021 Jun 10.
Published in final edited form as: Anal Chem. 2008 May 23;80(14):5616–5621. doi: 10.1021/ac8007016

Figure 2.

Figure 2.

Application of multiplexed fluorescence recovery detection. (a) Schematic representation of multiplexed fluorescence recovery detection. (b) Normalized kinetic fluorescence emission curves for the mixture of FAM (λex = 495 nm, λem = 515 nm, black line) released from Vit ERE and HEX (λex = 535 nm, λem = 555 nm, red line) released from Vit EREmt, respectively. (c) Normalized kinetic fluorescence emission curves for Cy5 (λex = 650 nm, λem = 670 nm, black line) released from VEGF and Texas red (λex = 585 nm, λem = 605 nm, red line) released from VEGFmt, respectively. (d) Normalized kinetic fluorescence emission curves of FAM (black line) released from Vit ERE and Cy5 (red line) released from VEGF.