. 2021 Jun 2;2021:5575260. doi: 10.1155/2021/5575260

Table 1.

Primer use in qPCR.

Gene target	Primer name	Oligonucleotide sequence	PCR product size (bp)	Ref
CAR gene	TestCarF	5′-TTTTACCCCT CCGACATCGC-3′	253
CAR gene	TestCarR	5′-CAGCACGATCAGAGCCATCTTG-3′	253

Human beta actin gene	actinF	5′-ATGAAGTGTGACGTGGACAT-3′	150	[21, 22]
Human beta actin gene	actinR	5′-CAGGAGGAGCAATGATCTTGAT-3′	150	[21, 22]

Cyclooxygenase 2 gene	COX2F	5′-CAAATGAGATTGTGGGAAAATTGCT-3′	300	[23]
Cyclooxygenase 2 gene	COX2R	5′-GATCATCTCTGCCTGAGTATCTT-3′	300	[23]

Calreticulin gene	CALRF	5′-AGTTCCGGCAAGTTCTACGG-3′	260	[24]
Calreticulin gene	CALRR	5′-CCACAGATGTCGGACCAAA-3′	260	[24]

Glyceraldehyde 3-phosphate dehydrogenase	GAPDHF	5′-TTGTCTCACTTGTTCTCT-3′	87	[25]
Glyceraldehyde 3-phosphate dehydrogenase	GAPDHR	5′-ATGGGAGTTGTTTTCTTG-3′	87	[25]