Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 8;39(6):737–746. doi: 10.1038/s41587-021-00815-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s), under exclusive licence to Springer Nature America, Inc. 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 5 — a, Selected genes, which were up- or downregulated in HFOs compared to cardiomyocytes generated by a conventional 2D differentiation protocol and respective gene functions according to the GeneCards database³⁸. FC= fold change. b, Cryosections of aggregates generated by a conventional 3D cardiac differentiation protocol stained for the cardiac markers NKX2.5, cTnT and sarcomeric actinin (SA). c, Long-term culture of HFOs. HES3 NKX2.5-eGFP-derived HFOs were cultured up to 146 days in suspension after dissolving the surrounding Matrigel. HFOs were stained with DiI-Ac-LDL to visualize endothelial cells. Scale bars: b: 100 µm; c: 500 µm.