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. 2021 Feb 8;39(6):737–746. doi: 10.1038/s41587-021-00815-9

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© The Author(s), under exclusive licence to Springer Nature America, Inc. 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Representative flow cytometry plot of an HFO stained for the endothelial cell (EC) marker CD31 (left) and EC amount in HFOs assessed by flow cytometry (right). Data are presented as mean ± SEM; n = 6 HFOs from 2 independent experiments. b, Paraffin section stained for CD31 and enlargements showing EC-lined cavities (dotted arrows) and a non-EC-lined cavity (arrowhead) in the inner core (IC) and ECs (solid arrow) between the IC and the myocardial layer (ML). c, Assessment of the average number of vessel-like structures (VL) present on paraffin sections of HFOs (front view). Left: Representative image of an HFO paraffin section stained for CD31, in which the VLs are marked with asterisks. Right: Number of VLs per 1 mm² IC area. Data are presented as mean ± SEM; n = 10 HFOs from 3 independent experiments. d, Cryosection stained for CD31 and NKX2.5 and enlargements showing CD31/ NKX2.5-double-positive endocardial-like cells (arrows) between the ML and the IC. (e) Multiphoton microscopy of an HFO. The figure shows still images of Supplementary Video 4 at different time points. Scale bars: b–d: 100 µm.