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(A) Treating established MRSA-1707 biofilms with HPs 3, 28, and 29 leads to a rapid induction of iron starvation. RT-qPCR results of HPs 3, 28, and 29 upregulating iron uptake genes in MRSA-1707 biofilms following 4 h treatment at (B) 1 μM or 1/10 × MBEC; *p value ≤ 0.05, **p ≤ 0.01 (Student’s T-test). (C) UV–vis spectroscopy of HPs 18, 28, and 29 binding iron(II). Note: In previous work, we show that EDTA and TPEN do not upregulate this panel of iron uptake genes at 5 μM.39
