. Author manuscript; available in PMC: 2022 Jun 10.

Published in final edited form as: J Med Chem. 2021 Apr 21;64(11):7275–7295. doi: 10.1021/acs.jmedchem.1c00168

Table 3.

Summary of Biofilm Eradication Studies against MRSA, MRSE, VRE, and E. faecalis Biofilms^a

compound	MRSA-1707 MBC/MBEC	MRSA 44 MBC/MBEC	MRSE 35984 MBC/MBEC	VRE 700221 MBC/MBEC	E. faecalis OG1RF MBC/MBEC	% hemolysis at 200 μM
3	50^c/100^c	37.5^b/75^b	25^c/50^c	18.8^b/12.5^c	25^c/>200^d	≤1
21	18.8^b/37.5^b					≤1
24	18.8^b/75^b					≤1
27	37.5^b/37.5^b					1.9
28	50/50	37.5^b/75^b	12.5^c/25^c	4.69^b/2.35^b	75^b/>200^d	≤1
29	1.56^c/2.35^b	4.69^b/6.25^c	1.17^b/0.59^b	0.59^b/0.59^b	6.25/18.8^b	≤1
30	18.8^b/4.69^b		3.13^c/2.35^b	0.78^c/0.30^b		≤1
31	75^b/75^b		4.69^b/1.17^b	2.35^b/0.20	1.56^c/0.78^c	27
32	9.38^b/2.35^b	4.69^b/6.25^c	1.56^c/1.56^c	0.78^c/0.39	18.8^b/75^b	2.6
33	12.5^c/18.8^b		12.5/12.5	3.13^c/1.17^b		≤1
34	2.35^b/3.13^c	6.25^c/6.25^c	0.59^b/0.30^b	0.39^c/0.20	3.13^c/1.17^b	≤1
35	4.69^b/4.69^b	6.25^c/18.8^b	2.35^b/2.35^b	0.59^b/0.30^b	6.25/6.25	≤1
36	12.5/12.5		6.25/6.25	4.69^b/0.59^b		5.1
37	12.5/9.38^b		6.25/6.25	4.69^b/3.13^c		3.0
45	50^c/200		12.5^c/4.69^b	9.38^b/3.13^c		5.7
49 (QAC-10)	93.8^b/93.8^b		3.13/3.13	3.0^b/3.0^b		>99
TPEN	375^b/>2000		250/>2000	188^c/>2000	500/>2000	≤1
EDTA	>2000/>2000		1000/>2000		>2000/>2000	≤1
Vancomycin	7.8/>2000	7.8/>2000	3.0^c/>2000	750^b/>2000	11.7^b/>2000
Daptomycin	125/>2000			375/93.8^b
Linezolid	31.3/>2000			4.69^b/1.56

All biological results are reported in micromolar (μM) concentrations.

Midpoint of a twofold range in observed values.

Midpoint of a fourfold range in values.

Partial turbidity observed at the highest test concentration.

All values in this table resulted from a minimum of three independent experiments. Halogenated phenazines and QAC-10 were tested at concentrations of up to 200 μM. Conventional antibiotics (e.g., vancomycin), EDTA, and TPEN (metal-binding agents) were tested at concentrations of up to 2000 μM in CBD assays.