A lack of carbachol-mediated increases in IPSC frequency and amplitude in CA1 PCs in IHK mice. (A, B) Current records in CA1 PCs before and during carbachol (10 μM) application made in ipsilateral hippocampal slices from a sham control or an IHK mouse. Note that carbachol caused an increase in frequency and amplitude of IPSCs in the CA1 PC from the sham control, but not from the IHK mouse. (C, D) Summary of frequency and amplitude of IPSCs before and during carbachol application. The numbers for sham (solid circles), IHK (ipsi; open circles), and IHK (contra; solid triangles) indicate the numbers of CA1 PCs tested for these experiments. Two-way ANOVA with repeated measures were followed by post hoc Tukey tests for mean comparisons. *, **, and *** indicate p < 0.05, p < 0.01, and p < 0.001 vs IHK ipsilateral, respectively; °, °°, and °°° indicate p < 0.05, p < 0.01, and p < 0.001 vs IHK contralateral, respectively.