Table 1.
List of reagents used for fluorescence microscopy
Reagent type | Product name | Manufacturer | Catalog number | Dilution |
---|---|---|---|---|
Primary antibody | Mouse anti-NeuN | Millipore | MAB377 | 1:100 |
Rabbit anti-GFAP | Abcam | Ab7260 | 1:500 | |
Guinea pig anti-CB1 | Frontier Institute Co. | CB1-GP-Af530 | 1:2,000 | |
Rabbit anti-Calbindin | Swant | CB-38a | 1:500 | |
Rabbit anti-vGAT | Synaptic Systems | 131003 | 1:1,000 | |
Rabbit anti-proCCK | Frontier Institute Co. | CCK-pro-Rb-Af350 | 1:1,000 | |
Mouse anti-GABAB1R | Abcam | Ab55051 | 1:5,000 | |
Secondary antibody | Goat anti-mouse IgG (H+L), AF 488 | Invitrogen | A11001 | 1:500 |
Goat anti-mouse IgG2a, AF 555 | Invitrogen | A21137 | 1:500 | |
Goat anti-mouse IgG (H+L), AF 594 | Invitrogen | A11005 | 1:500 | |
Goat anti-rabbit IgG (H+L), AF 488 | Invitrogen | A11008 | 1:500 | |
Goat anti-rabbit IgG (H+L), AF 594 | Invitrogen | A11012 | 1:500 | |
Goat anti-rabbit IgG (H+L), CF 633 | Biotium | 20122 | 1:500 | |
Goat anti-Guinea Pig IgG (H+L), AF 488 | Invitrogen | A11073 | 1:500 | |
Other | DAPI | Invitrogen | D1306 | 1:1,000 |
Rhodamine Red-X (RRX) Streptavidin | Jackson ImmunoResearch | 016-290-084 | 1:500 |