Table 2.
The effects of exposure to nicotine and ethanol, either alone or simultaneously, during in utero life and breastfeeding periods on the averages of Johnsen’s score and seminiferous tubules diameter and area in the testicular tissue of F1 adult pups.
| Groups | Seminiferous tubule diameter (μm) | Seminiferous tubule area (µm)2 | Johnsen’s score |
|---|---|---|---|
| Veh | 77.24 ± 0.83 | 50.985 ± 0.51 | 9.66 ± 0.04 |
| Nicotine | 67.64 ± 1.90a** | 46.69 ± 2.53 | 8.98 ± 0.09a*** |
| Ethanol | 69.59 ± 1.99a* | 46.29 ± 3.38 | 9.24 ± 0.08a** |
| Nic + Ethn | 72.93 ± 1.96 | 52.17 ± 1.42 | 9.23 ± 0.08a** |
| Statistics | F(3,18) = 4.97, p = 0.01 | F(3,18) = 1.61, p = 0.23 | F(3,18) = 19.38, p = 0.000 |
Superscript letter (a) indicates a significant difference compared to the vehicle group. *, ** and *** indicate p ≤ 0.05, p ≤ 0.01 and p ≤ 0.001, respectively. Data present Mean ± SEM.