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. 2021 Jun 10;12(6):602. doi: 10.1038/s41419-021-03857-8

Fig. 2. FKBP4 activates NF-κB through IKKβ.

Fig. 2

A Heat map of the normalized relative abundance levels of IKK/NF-κB pathway-related genes significantly changed between the control and FKBP4-knockdown groups. These key genes positively (or negatively) regulated IKK/NF-κB signaling and NF-κB transcriptional activity decreased (or increased) after FKBP4 inhibition. B Western blot analysis of IKK/NF-κB signaling molecules in PC9 and H1975 cells transfected with FKBP4 shRNA. C Western blot analysis of IKK/NF-κB signaling molecules in BEAS-2B cells overexpressing FKBP4. D Inhibition of FKBP4 attenuated TNF-α-induced NF-κB activity in H1975 cells, while overexpression of FKBP4 promoted TNF-α-induced NF-κB activity in BEAS-2B cells. These cells were stimulated with 10 ng/ml TNF-α for 6 h. The transcriptional activity of NF-κB was measured with a luciferase reporter and normalized to Renilla activity. E FKBP4 promotes NF-κB transcriptional activity through IKKβ. Luciferase reporter assays of H1975, BEAS-2B, and 293T cells cotransfected with FKBP4 shRNA (or FKBP4) and IKKβ plasmids were performed as indicated (*P < 0.01, **P < 0.01, ***P < 0.001).