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. 2021 Jun 10;12(6):602. doi: 10.1038/s41419-021-03857-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A–E PC9 and H1975 cells were transiently transfected with FKBP4 or negative control siRNAs. A Western blot analysis of successful FKBP4 knockdown in PC9 and H1975 cells. The effects of FKBP4 knockdown on cell proliferation were detected by CCK8 assay (B) and colony-formation assay (C). D Effects of FKBP4 knockdown on the cell cycle were detected by flow cytometry. E Effects of FKBP4 knockdown on metastasis and invasion were detected by Transwell assays ± Matrigel. F–J BEAS-2B cells were transiently transfected with FKBP4 or negative control plasmids. F Western blot analysis of successful FKBP4 overexpression in BEAS-2B cells. Effects of FKBP4 overexpression on cell proliferation were detected by CCK8 assay (G) and colony-formation assay (H). I Effects of FKBP4 overexpression on the cell cycle were detected by flow cytometry. J Effects of FKBP4 overexpression on metastasis and invasion were detected by Transwell assays ± Matrigel.