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. 2021 Jun 10;12(6):601. doi: 10.1038/s41419-021-03847-w

Fig. 1. Optimization of the protocol to induce acute pancreatitis in vivo.

Fig. 1

A Western blot of MLKL expression in splenocytes from Mlkl+/− and Mlkl−/− mice by using antibodies recognizing the C- and N-terminal domains of MLKL. Mlkl+/− and Mlkl−/− mice received intraperitoneal injection of three doses of caerulein, 50 µg/kg/body weight at 8 h intervals, 50 µg/kg/body weight at 6 h intervals for 2 days, and 100 µg/kg/body weight at 8 h intervals. Twenty-four hours after the first injection, mice were sacrificed (n = 5−10 in each group). B Representative haematoxylin and eosin (H&E) images of pancreases from Mlkl+/− and Mlkl−/− mice treated with or without caerulein. C Total weight gain (grams), D pancreas weight gain (gram), and E the ratio of pancreatic weight to the final body weight (milligrams to gram) were determined. F Representative H&E images of pancreases from male and female Mlkl−/− mice after receiving caerulein for 8 h. Data are present as means ± SE. *P < 0.05, **P < 0.01, and ***P < 0.001.